Statistical Distributions of Pyrosequencing

Statistical distributions of pyrosequencing

Pyrosequencing is emerging as one of the important next-generation sequencing technologies. We derive the statistical distributions of this technique in terms of nucleotide probabilities of the target sequences. We give exact distributions both for fixed number of flow cycles and for fixed sequence length. Explicit formulas are derived for the mean and variance of these distributions. In both c...

Distributions of positive signals in pyrosequencing

Pyrosequencing is one of the important next-generation sequencing technologies. We derive the distribution of the number of positive signals in pyrograms of this sequencing technology as a function of flow cycle numbers and nucleotide probabilities of the target sequences. As for the distribution of sequence length, we also derive the distribution of positive signals for the fixed flow cycle mo...

Statistical Distributions, Second Edition

advantage of the unique capabilities of the computer to teach. The self-test segment of the program is simply a memory game, which does not focus on clinically relevant anatomy or provide a framework on how to think radiologically. In summary, while The Imaging Atlas functions appropriately, it offers few advantages over a radiographic atlas in book form. A firm grasp of statistical distributio...

1 Statistical Distributions

Statistical tools for ultra-deep pyrosequencing of fast evolving viruses

We aim to detect minor variant Hepatitis B viruses (HBV) in 38 pyrosequencing samples from infected individuals. Errors involved in the amplification and ultra deep pyrosequencing (UDPS) of these samples are characterised using HBV plasmid controls. Homopolymeric regions and quality scores are found to be significant covariates in determining insertion and deletion (indel) error rates, but not ...